Regulatory

Part:BBa_K264042:Design

Designed by: Maria McClintock   Group: iGEM09_Virginia_Commonwealth   (2009-10-21)


K264001.J23101 (UP/promoter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 31
    Illegal NheI site found at 54
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A spacer, CT, was placed between the UP element and promoter. There is no scar between the UP element and promoter.


Source

This part is a combination of UP element design 2 and the constitutive promoter BBa_J23101.

References